Regulation of Normal Human Blood Neutrophilic , Macrophagic , and Eosinophilic Committed Stem Cell Proliferation by Autologous Blood T Lymphocyte

The influence of T lymphocyte (T cell) subsets on the proliferation of the blood neutrophilic. macrophagic. and eosinophilic cluster and colony (aggregate) forming cells (CFC) was evaluated. The T cells and the CFC-enriched Bnull cells (mononuclear cells depleted of monocytes and T cells) and null cells (Bnull cells depleted of B lymphocytes) were separated from the blood of normal individuals. The population enriched for OKT4 (T4 cells) monoclonal antibody-binding T helper/inducer cells and for OKT8 (T8 cells) monoclonal antibody-binding T suppressor/cytotoxic cells were separated from the T cells or mononuclear cells depleted of monocytes by negative enrichment using an immunoadherence ‘panning” method. These separated cell populations were cultured separately. and the Bnull or null cells were cocultured with the T. T4. and T8 cells in agar medium in the presence and absence of an exogenous source of colony-stimulating activity (CSA). The cultures